We are looking for a full-time highly motivated, enthusiastic, and detail-oriented laboratory research assistant for a minimum 2-year commitment to study the genetic determinants of human neurological diseases with a special focus on epilepsy. The scope of our research activities broadly includes working with human brain tissue, cell culture experiments, and mouse modeling work. There will be opportunities for independent projects with the expectation that the RA will contribute to peer-reviewed publications. The RA will be directly supervised and reports to Dr. Sattar Khoshkhoo, and will be part of a larger research group of Dr. Chris Walsh at Boston Children's Hospital. The experience is uniquely suited to students pursuing graduate school or a career at the biomedical translational interface. Candidates who plan to pursue PhD or MD/PhD programs will be given preference.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
- Takes an active role in next-generation sequencing experiments including DNA and RNA extraction, single cell DNA and RNA sequencing, and next-generation sequencing library preparation, and variant validation.
- Participates in a range of experiments using frozen human brain tissue including immunostaining, nuclear extraction, flow cytometry.
- Performs bacterial cloning experiments.
- Maintains human and mouse primary and immortalized cell lines and performs cell culture experiments including transfection, imaging, and multi-electrode array recordings.
- Maintains mouse colony and assists with mouse surgeries including in utero electroporation and EEG implantation.
- Contributes to general lab maintenance including cleaning, organization, maintaining inventory, and ordering supplies.
- Helps with basic analysis of next-generation sequencing data.
- Assists on drafting study guidelines and protocols.
- Consents potential study patients and coordinates collection of tissue from the operating room.
- Keeps detailed records of clinical and sample information.
- Prepares and submits all IRB documents; applications, amendments, annual reviews and serious adverse events.
- Maintains all study regulatory documents.
- Provides general research administrative support and trains other staff.
- BS or BA with relevant coursework
- Preference given to candidates with 1+ year of prior molecular biology, genetics, or neuroscience research lab experience.
- Quantitative skills and familiarity with a programming language such as R or Python is not required but is helpful.
- Excellent interpersonal skills and ability to work in a team.
- Good oral and written communication skills.
- Able to work independently.
- Ability to problem solve quickly and accurately.
- Excellent organizational skills and ability to prioritize and delegate a variety of tasks.
- Careful attention to detail.
- Ability to demonstrate professionalism.
- The candidate will be hired through Brigham and Women's Hospital, but initially spend over 80% of their time in a laboratory research environment at Boston Children's Hospital in the Division of Genetics and Genomics. The remainder of their time will be spent at Brigham and Women's Hospital, predominantly in the clinical research setting.
BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.
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